|
KMID : 0613820060160010076
|
|
Journal of Life Science
2006 Volume.16 No. 1 p.76 ~ p.81
|
|
|
In vivo Functional Analysis of γ-Butyrolactone Autoregulator Receptor Gene (scaR) in Streptomyces clavuligerus
|
|
Kang Soo-Jin
Lee Chang-Kwon
Choi Sun-Uk
Kim Hyun-Soo
Hwang Yong-Il
|
|
|
Abstract
|
|
|
|
A γ-butyrolactone autoregulator receptor has a common activity as DNA-binding transcriptional repressors controlling secondary metabolism and/or morphological differentiation in Streptomyces. A gene (scaR) encoding it was cloned from Streptomyces clavuligerus, a clavulanic acid producer, and was in vitro characterized in a previous report. In this study to clarify the in vivo function of ScaR, a γ-butyrolactone autoregulator receptor of Streptomyces clavuligerus, we constructed a scaR-deleted strain by means of homologous recombination. No difference in morphology was found between the wild-type strain and the scaR-disruptant, but the scaR-disruptant showed higher clavulanic acid production. This indicates that the ScaR in S. clavuligerus acts as a negative regulator of the biosynthesis of clavulanic acid, but plays no role in morphological differentiation.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|